Quantitative Study of the Structural Requirements of Phthalazine/Quinazoline Derivatives for Interaction with Human Liver Aldehyde Oxidase
スポンサーリンク
概要
- 論文の詳細を見る
Aldehyde oxidase is a molybdenum-containing enzyme distributed throughout the animal kingdom. Although this enzyme is capable of metabolizing a wide range of aldehydes and N-heterocyclic compounds, there is no reported detailed study of physicochemical requirements of the enzyme-substrate interactions. The aim of this study, therefore, was to investigate quantitatively the relationships between the kinetic constants of aldehyde oxidase-catalyzed oxidation of some phthalazine and quinazoline derivatives (as substrates) and their structural parameters. Multiple regression and stepwise regression analyses showed that polarity of phthalazines (expressed as dipole moment μ, cohesive energy density δT and an indicator variable for hydrogen-bond acceptor ability of R1 substituent, HBA) had a negative effect on the enzyme activity (leading to the reduction of V_<max> and increase of K_m). Electron withdrawing substituents in the quinazoline series are favorable for interaction with the enzyme. This finding and also the relationships of 1/K_m of phthalazines with the energy of the lowest unoccupied molecular orbital and log V_<max>/log K_m of phthalazines with degree of bonding of the two nitrogen atoms in the molecules are consistent with the mechanism of action. The reaction involves a nucleophilic attack on an electron-deficient sp^2-hybridized carbon atom and formation of an epoxide intermediate following the disruption of the aromatic structure.
- 公益社団法人日本薬学会の論文
- 2001-09-01
著者
-
Rashidi Mohammad
Department Of Medicinal Chemistry School Of Pharmacy Tabriz Medical Sciences University
-
GHAFOURIAN Taravat
Department of Medicinal Chemistry, School of Pharmacy, Tabriz Medical Sciences University
-
Ghafourian Taravat
Department Of Medicinal Chemistry School Of Pharmacy Tabriz Medical Sciences University