Simultaneous Determination of Chlorogenic Acid, Caffeic Acid, Ferulic Acid, Protocatechuic Acid and Protocatechuic Aldehyde in Chinese Herbal Preparation by RP-HPLC

概要

論文の詳細を見る
In the present study, a reversed phase high performance liquid chromatographic (RP-HPLC) method was established for simultaneous determination of chlorogenic acid, caffeic acid, ferulic acid, protocatechuic acid and protocatechuic aldehyde in a Chinese herbal preparation (Fufang-Pugongying-Mixture). The separation was performed on a Hypersil ODS-2 column by isocratic elution with methanol and 0.2м acetate buffer (pH 3.6) (15:85, v/v) as the mobile phase at the flow-rate of 1.0ml/min with operating temperature of 30℃, and detection wavelength of 300 nm. A good linear regression relationship between peak-areas and concentrations was obtained over the range of 2-200μg/ml for the five marker compounds mentioned above. The spike recoveries were within 96.72-104.07%. The variation coefficient (CV) values of the precision were in the range of 0.89-4.50%. Moreover the developed method has reference value for quantitative analysis of Taraxacum, Lonicera and Angelica.
公益社団法人日本薬学会の論文
2004-10-01