Zic1 is a Transcriptional Represser Through the Lamin A/C Promoter and has an Intrinsic Repressive Domain

概要

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Zic family proteins are expressed in the cerebellum and play important roles in vertebrate development. However, very few observations have been made regarding the function of Zic proteins in transcription. We previously showed, using P19 cells as a model system, that the lamin A/C promoter has a retinoic acid responsive element (L-RARE), and that Spl and Sp3 bind the CACCC box of the L-RARE. Here, Zicl is identified as a binding protein of the L-RARE by yeast one-hybrid screening. We show that Zic1 can bind to and repress transcription through the CACCC box of the L-RARE. Moreover, reporter assays using various fusion proteins of Zicl with the GAL4 DNA binding domain reveal that Zic1 has an intrinsic transcriptional repressive and activation domain.
公益社団法人日本薬学会の論文
2004-08-01