Epitope-Tagged Metallothionein : Its Biochemical and Immunocytochemical Applications

スポンサーリンク

概要

• 論文の詳細を見る

• We developed epitope-tagged metallothionein IIA cDNA by the addition of an 8-amino acid sequence, DYKDDDDK, at the N-terminus metallothionein using polymerase chain reaction. The fusion proteins were expressed by transfection in human embryonic kidney 293 (HEK-293) cells and were specifically recognized by commercially available antibody, an anti-Flag M2 monoclonal antibody. Furthermore, the fusion protein was detectable on a Western blot, whose migration was expectedly at 7000-8000 in an SDS-denatured form. Thus, availability of the epitope-tagged metallothionein will facilitate the investigation of cellular mechanisms of the protein such as its nuclear localization mechanism.

• 公益社団法人日本薬学会の論文
• 2003-04-01

著者

• Ishido Masami

  Division Of Environmental Health Sciences National Institute For Environmental Studies

• Sayato-Suzuki Junko

  Division of Environmental Health Sciences National Institute for Environmental Studies

関連論文

• Epitope-Tagged Metallothionein : Its Biochemical and Immunocytochemical Applications

スポンサーリンク