In Vitro Propagation by Asymbiotic Seed Germination and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) Radical Scavenging Activity Studies of Tissue Culture Raised Plants of Three Medicinally Important Species of Dendrobium (Medicinal Chemistry)

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概要

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• A simple and efficient plant propagation system has been developed by asymbiotic germination of seeds in three medicinally important Dendrobium species, namely, Dendrobium tosaense, Dendrobium moniliforme, and Dendrobium linawianum. Plants obtained from natural habitats were grown in the greenhouse. The flowers were hand pollinated. Seeds of the capsules derived after 12 weeks of hand-pollination germinated asymbiotically (50-74%) on half strength Murashige and Skoog's (MS) basal medium with 3% sucrose and solidified with 0.9% Difco agar. Active growth in the germinated seedlings was achieved by re-culturing on full strength MS basal medium supplemented with 8% banana homogenate, 8% potato homogenate, 8% coconut water, 1.5% sucrose and 0.9% Difco agar. Healthy plantlets, transferred to plastic trays containing moss or moss and tree fern, successfully acclimatized (84-100%) in the greenhouse. A marked varied response was observed in the free radical scavenging activity of methanolic extracts of in vitro propagated plants, on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical using a UV spectrophotometer assay. Methanolic extracts were prepared by dissolving the powdered plant material, obtained from six months old in vitro propagated plants, each about 5g, in boiling methanol. The percentage of scavenging effect of D. tosaense extract was 95.9% at 0.4 mg/ml concentration, whereas D. monoliforme, and D. linawianum extracts scavenged 83.4% and 92.3%, respectively, at a concentration of 0.4 mg/ml. All the extracts scavenged DPPH radical significantly in a concentration dependent manner.

• 公益社団法人日本薬学会の論文
• 2004-05-01

著者

• Kuo C‐l

  Institute Of Chinese Pharmaceutical Science China Medical University

• Tsay H‐s

  Institute Of Biotechnology Chaoyang University Of Technology

• Nalawade Satish

  Institute Of Biotechnology Chaoyang University Of Technology

• LO Shu-Fung

  Department of Agronomy, Chiayi Agricultural Experiment Station, Agricultural Research Institute

• MULABAGAL Vanisree

  Institute of Biotechnology, Chaoyang University of Technology

• MATTEHEW Susan

  Institute of Chinese Pharmaceutical Science, China Medical University

• CHEN Chung-Li

  Department of Agronomy, National Chung Hsing University

• KUO Chao-Lin

  Institute of Chinese Pharmaceutical Science, China Medical University

• TSAY Hsin-Sheng

  Institute of Biotechnology, Chaoyang University of Technology

• Lo Shu-fung

  Department Of Agronomy Chiayi Agricultural Experiment Station Agricultural Research Institute:depart

• Chen Chung-li

  Department Of Agronomy National Chung Hsing University

• Mattehew Susan

  Institute Of Chinese Pharmaceutical Science China Medical University

• Tsay Hsin-sheng

  Institute Of Biotechnology Chaoyang University Of Technology

• Mulabagal Vanisree

  Institute Of Biotechnology Chaoyang University Of Technology

関連論文

• Micropropagation of Polygonum multiflorum THUNB and Quantitative Analysis of the Anthraquinones Emodin and Physcion Formed in in Vitro Propagated Shoots and Plants(Miscellaneous)
• Improving Effects of Epigallocatechin-3-Gallate on Hemorheological Abnormalities of Aging Guinea Pigs
• In Vitro Propagation by Asymbiotic Seed Germination and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) Radical Scavenging Activity Studies of Tissue Culture Raised Plants of Three Medicinally Important Species of Dendrobium (Medicinal Chemistry)
• Isolation and Quantitative Analysis of Cryptotanshinone, an Active Quinoid Diterpene Formed in Callus of Salvia miltiorrhiza BUNGE

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