Characterization and Evolution of a cDNA Encoding the Small Subunit of Ribulose-1, 5-bisphosphate Carboxylase/oxygenase (rbcS) of Stellaria longipes (Caryophyllaceae)

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A cDNA clone encoding the small subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase (rbcS) of Stellaria longipes (family Caryophyllaceae) was isolated and sequenced. This cDNA was used as a probe to investigate the gene complexity and expression among different genotypes. Southern blot analysis showed that the S. longipes genome contained a rbcS gene family. A higher level of rbcS mRNA was detected in the genotype grown under the long-day photoperiod and warm condition than under the short-day photoperiod and cold condition, suggesting the effect of growth condition on the gene expression. Phylogenetic analysis of both the nucleotide and deduced amino acid sequences for both the signal peptide and the mature protein, showed that among ten families of higher plants tested (including four monocot, 14 dicot and one gymnosperm taxa), the rbcS of S. longipes. along with another member of Caryophyllaceae Silene pratensis, was more closely related to the families Solanaceae, Brassicaceae and Fabaceae than it was to other plant families.
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