Oviduct 17β-Estradiol Receptor in the Female Lizard, Podarcis s. sicula, during the Sexual Cycle : Relation to Plasma 17β-Estradiol Concentration and Its Binding Proteins(Endocrinology)

概要

論文の詳細を見る
In the oviparous lizard, Podarcis s. sicula, 17β-estradiol binding molecules were characterized in the oviduct and plasma. In addition, their concentration was evaluated throughout the annual cycle. In the oviduct, a 17β-estradiol receptor (ER) is present. It shows high affinity for ligand (4.9-7.0×10^<-10> M). It is decreased by ovariectomy, and induced by 17β-estradiol treatment. 17β-estradiol causes also a ER shift from the cytosol into the nuclei. On sucrose gradient ER behaviour is consistent with the properties of ER obtained from other 17β-estradiol target organs. At isoelectrofocusing the labeled 17β-estradiol binding molecules fall into three pH ranges: 5.2-5.6, 7.0-7.7, 8,0-8.7. Nuclear filled ER significantly increases as oviduct grows. This supports an ER role in oviduct stimulation by 17β-estradiol. In the plasma, 17β-estradiol binding molecules show many physico-chemical and behavioural properties of steroid-binding proteins (SBPs).
社団法人日本動物学会の論文
1992-10-15