Antibody Production in the Goat : Immunokinetics and Epitope Specificity Using a Glycoprotein Immunogen(Immunology)

概要

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We have developed methods for the efficient production of antibodies in goats using a glycoprotein purified from the eggs of the frog Xenopus laevis. When emulsified with complete Freund's adjuvant, the amount of immunogen required to elicit high liter antiserum was much less than commonly used (50 μg as opposed to mg quantities). A single immunizing injection produced a maximum antibody response and secondary immunizations were not beneficial. The IgG was directed toward the carbohydrate moiety when the glycoprotein was used as immunogen, although the protein moiety was immunogenic as shown by the use of the deglycosylated polypeptide. Goats immunized with glycosylated immunogen all produced maximum titer antisera from 35 to 45 days postimmunization. In contrast, maximum titer in goats injected with deglycosylated polypeptide was reached at 26 days postimmunization. We anticipate that the immunization methods reported here will improve the usefulness of the goat for preparing polyclonal antibodies.
社団法人日本動物学会の論文
1992-10-15