The Confocal Laser Scanning Microscope as a Tool for Studying Xanthophores of the Swordtail(Xiphophorus helleri)(COMMUNICATION)(Cell Biology)

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概要

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• The morphology of xanthophores on scales of the swordtail, Xiphophoms hellerl, was studied using bright-field light, differential interference contrast (DIG) and confocal laser scanning microscopies (CLSM). Under light microscopy, xanthophores were indistinct with diffused cell outline. Improved imaging, showing gradient shaded relief-like images of xanthophores, was obtained under DIG. The CLSM produced the clearest images of xanthophores and also intracellular details as these cells were autofluorescent when scanned with the argon ion laser beam. The cell nuclei were non-autofluorescent and thus appeared dark. Selected portions of a cell can be highly magnified to show intracellular structures using the CLSM computer software. Also, serial optical tomographic sections of xanthophores with the CLSM enabled studies of intracellular structures present at different depths of the cells, thus permitting reconstruction of three-dimensional cell models. Therefore, use of the CLSM is highly recommened to complement bright-field light and DIG microscopies for morphological studies of xanthophores and other autofluorescent cells.

• 社団法人日本動物学会の論文
• 1992-06-15

著者

• Phang V.

  Department Of Zoology National University Of Singapore

• Phang V.p.e.

  Department Of Zoology National University Of Singapore

• LIM T.

  Department of Medicine, National University of Singapore

• Khoo G.

  Department Of Zoology National University Of Singapore

• Lim T

  National Univ. Singapore Kent Ridge Sgp

• Lim T.

  Department Of Medicine National University Of Singapore

• Lim T.M.

  Department of Zoology, National University of Singapore

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