The Sporadic Change of Epiblast Cells to Epithelial in the Chick Embryo before the Formation of Hensen's Knot(Developmental Biology)

概要

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33 chick embryos from stage 2 to stage 4 according to Hamburger and Hamilton [1] were stained in toto with Rhodamine phalloidin (Rh-P) and then surveyed with a fluorescence microscope. Two types of cells were stained: one, elongated and stained along a whole cell surface, another, not elongated, stained eminently along the polygonal cell border at the upper surface. The former, recognized at or along the growing primitive streak (PS), were considered as the mesodermal cells because of their flask-like shape and downward inclination. The latter, appearing at the later stage of PS formation, were considered as the epithelial cells because of the polygonal frame work of F-actin along the cell border. At first, a few epithelial cells clustered to form small islands scattering along and slightly apart from the PS. The islands then increased in both number and size, becoming connnected to each other forming two belts along the PS. When Hensen's knot was formed, all epiblastic cells differntiated into epithelial cells with the polygonal framework of F-actin.
社団法人日本動物学会の論文
1991-10-15