A Rapid and Sensitive In Vitro Bioassay of Follicle Stimulating Hormone: Estradiol-17β Formation by Dispersed Seminiferous Tubule Cells from Immature Rats(Endocrinology)

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概要

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• The aromatase activity of Sertoli cells enriched cultures from immature rat testis has recently been utilized for in vitro bioassay of follicle stimulating hormone (FSH), with conversion of exogenous 19-hydroxyl-androstenedione (19OH-A) to estradiol-17β Being used as end point. The process of the preparation of cultured Sertoli cells was, however, rather lengthy. We have simplified the bioassay system, by directly employing the dispersed seminiferous tubule cells, without initial culture of the Sertoli cells for 2-4 days, for incubation with FSHs. Such simplified incubation system was found to be rapid, sensitive and specific for in vitro bioassay of purified FSH preparations. In brief, decapsulated testes, obtained from 12 days old rats, were dispersed with collagenase for 10 min in Medium A. The dispersed seminiferous tubule cells were distributed into the multiwell of culture plate, preincubated with 19OH-A for 2 hr and then incubated with varying doses of FSHs in Medium B (Mediums A+0.125 rnM MIX) at 25℃ for 20 hr, under continuous aeration in Dubnoff incubator shaken at 50 cycle/min. Dose related estradiol-17β productions were produced by various mammalian FSHs. Such simplified incubation system is highly reliable and useful for in vitro bioassay of FSH activity from purified hormonal preparations or for monitoring FSH activity during isolation of the hormones. Cyclic AMP formation and accumulation by the dispersed seminiferous tubule cells were also investigated. The results indicated that the estradiol-17β Formation system is more sensitive than the cyclic AMP production system for in vitro FSH bioassay.

• 社団法人日本動物学会の論文
• 1991-08-15

著者

• Yu John

  Endocrinology Laboratory Institute Of Zoology Academia Sinica

• SHEN SAN

  Endocrinology Laboratory, Institute of Zoology, Academia Sinica

• Shen San

  Endocrinology Laboratory Institute Of Zoology Academia Sinica

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• A Rapid and Sensitive In Vitro Bioassay of Follicle Stimulating Hormone: Estradiol-17β Formation by Dispersed Seminiferous Tubule Cells from Immature Rats(Endocrinology)

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