Concanavalin A-dorsalized Ventral Mesoderm Mimics the Neural-inducing Activity of the Organizer in Cynops Embryo(Developmental Biology)

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概要

• 論文の詳細を見る

• The neural-inducing activity of ventral mesoderm aged after Con A treatment was examined in detail. To determine the time at which this function was attained, Con A-treated ventral mesoderm was incubated at different time lapses and sandwiched between competent ectoderm explants. Neural-inducing activity was found have been acquired as early as about 15 hr after Con A treatment and was maximal about 24 hr after Con A treatment. Brain, spinal cord and small neural tissues were observed. Oligomannose-type oligosaccharide, a specific ligand of Con A, failed to inhibit dorsalization one hour after Con A treatment. This possibly hints that the ventral mesoderm might have been determined to proceed to dorsal differentiation about one hour from application of Con A. Autoradiographic observation showed accumulation of ^<125>I-Con A on or around the nucleus 24 hr after Con A treatment. This may suggest a possible role of the nucleus in the acquisition of neural-inducing activity of Con A-dorsalized ventral mesoderm.

• 社団法人日本動物学会の論文
• 1991-08-15

著者

• Takahashi T

  Radioisotope Research Center Nagoya University

• Takata Kenzo

  Department Of General Education Doho University

• Takeshima Kazuhito

  Radioisotope Research Center, Nagoya University

• DIAZ MARIA

  Department of Biology, School of Science

• TAKAHASHI TADASHI

  Radioisotope Research Center, Nagoya University

• Diaz Maria

  Department Of Biology School Of Science

• Takeshima Kazuhito

  Radioisotope Research Center Nagoya University

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• Concanavalin A-dorsalized Ventral Mesoderm Mimics the Neural-inducing Activity of the Organizer in Cynops Embryo(Developmental Biology)
• Expression of the Epidermis-specific Gene ep37 before and after Metamorphosis of the Japanese Newt, Cynops pyrrhogaster
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