The Involvemnt of Phosphoenolpyruvate Carboxykinase in Succinate Formation in Phascolosoma arcuatum (Sipuncula) Exposed to Environmental Anoxia(Physiology)

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概要

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• Succinate, propionate and alanine were accumulated in the sipunculid worm, Phascolo-soma arcuatum, with a decrease in aspartate content upon anoxic exposure. Aspartate degradation could not account for all the succinate accumulated during the 48 hr anoxic period and no stoichiometric relationship between alanine accumulation and aspartate degradation was observed. The glutamate content in the worm remained constant throughout the 48 hr period of anoxia. Adenylosuccinate synthetase and adenylosuccinate lyase were not detected in P. arcuatum, rendering the formation of succinate through the purine nucleotide cycle in this organism improbable. Results indicate that succinate synthesis in P. arcuatum involved phosphoenolpyruvate carboxykinase at the phosphoenol-pyruvate branch point of glycolysis. The activity of phosphoenolpyruvate carboxykinase from P. Arcuatum exposed to anoxia remained unchanged throughout the 48 hr period, but the activity of pyruvate kinase measured at a subsaturating concentration of phosphoenolpyruvate decreased, possibly due to deactivation by phosphorylation. After 36 hr of anoxic exposure, the activity ratio of pyruvate kinase: phosphoenolpyruvate carboxykinase, obtained at 0.1 mM of phosphoenlopyruvate, decreased to half of the control value. Furthermore, the formation of oxaloacetate, and hence succinate, through phosphoenolpyruvate carboxykinase was favoured by the combined effects of lower pH and increased bicarbonate and alanine contents in the worm exposed to anoxia.

• 社団法人日本動物学会の論文
• 1991-08-15

著者

• IP YUEN

  Departuent of Zoology, National University of Zoology, Kent Ridge

• Ip Yuen

  Department Of Biological Sciences National University Of Singapore

• LIM RITA

  Department of Zoology, National University of Singapore

• Lim Rita

  Department Of Zoology National University Of Singapore

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