Oestradiol-17β Affects differentially Viability, Progesterone Secretion, and Apical Surface Morphology of Hamster Ovarian Follicles in vitro(Endocrinology)

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Previous studies have shown that oestradiol-17β (OE_2) exerts profound stimulatory effects on rat granulosa cells (GC) in vivo and in vitro while exerting an atretogenic effect on ovarian follicles of monkeys. We wished to determine the effects of OE_2 on a model intermediate between in-vivo animal and in-vitro cell studies, that of explanted hamster follicles in vitro. Hamsters were sacrificed on the morning of proestrus, ovaries were removed, and preovulatory follicles were excised and placed in culture in the presence or absence of OE_2. Following culture, GC and oocyte viability were assessed. Additionally, culture media were collected at 24-hr intervals and analyzed for progesterone (P). Follicles remained viable by most indices. There was a slight increase in GC viability at 72 hr with 1μg OE_2/ml. P accumulation was likewise transiently increased in the treated group at only 24 hr. Scanning electron microscopy, however, revealed that OE_2 treatment dramatically altered surface epithelial cells by increasing blebbing. The present study suggests that OE_2, at best, exerts only mildly stimulatory effects on viability and steroidogenesis of whole hamster follicles in vitro; this is comparable to effects seen in hamster GC, and in vivo, but is in stark contrast to the exaggerated stimulatory responses observed for the rat. Major changes in epithelial cell surface may designate this as a locus of OE_2 effects. We expect that this model will serve as a more physiologic paradigm than other in-vitro systems in the analysis of direct ovarian effects of estrogens and other bioactive molecules.
社団法人日本動物学会の論文
1991-02-15

Oestradiol-17β Affects differentially Viability, Progesterone Secretion, and Apical Surface Morphology of Hamster Ovarian Follicles in vitro(Endocrinology)

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