Identification of Acid Mucopolysaccharides in Summer Cells in Adrenal Glands of the Frog Rana catesbeiana(Endocrinology)

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概要

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• Acid mucopolysaccharides (MPS) within the cytoplasmic granules of the "summer cell" in the adrenal gland of Rana catesbeiana were analyzed by means of histochemistry and electrophoresis. Metachromasia with toluidine blue and alcian blue staining at low pH ranges showed the existence of acid MPS in high concentrations. Based on methylation-saponification experiments, they were considered to be sulfated MPS. Determination of the metachromatic peak wavelength by microspectrophotometry and alcian blue staining under varying ionic conditions (shift of pH, critical electrolyte concentration technique and ion-association technique with cetylpyridinium chloride) showed that the acid MPS in the summer cells are less sulfated than heparin. Two-dimensional electrophoresis of MPS on cellulose acetate membranes revealed the existence of a heparitin sulfate-like component and chondroitin sulfate-like ones. It was concluded that two kinds of acid MPS exist in the cytoplasmic granules of summer cells. The results of nitrous acid treatment, PAS reaction and chondroitinase digestion supported this conclusion.

• 社団法人日本動物学会の論文
• 1985-10-15

著者

• Kawamura Kosuke

  Zoological Institute Faculty Of Science University Of Tokyo:(present Address)department Of Biology S

関連論文

• Identification of Acid Mucopolysaccharides in Summer Cells in Adrenal Glands of the Frog Rana catesbeiana(Endocrinology)

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