Immunocytochemical Localization of Retinochrome in the Visual Cells of the Squid(Cell Biology)

概要

論文の詳細を見る
The intracellular location of a photosensitive membrane protein, retinochrome, in the visual cells of the squid Todarodes was investigated using immunocytochemical techniques. Fluorescent antibody techniques revealed that retinochrome was localized in the basal regions of the outer segments and throughout the cytoplasm of the inner segments. The distribution pattern of the immunofluorescence coincided with that of the myelin-like membrane structures called myeloid bodies, suggesting that retinochrome might be located in the myeloid bodies. To confirm this, a membrane homogenate containing the myeloid bodies was prepared from outer segment-free retinas, incubated with T4-phagelabeled antibody and examined with a scanning electron microscope. A number of phage particles were found to adhere to the surface of the membranes of the myeloid bodies. It was clear that the myeloid bodies carry retinochrome in the inner segments of the visual cells.
社団法人日本動物学会の論文
1985-06-15