Epidermal Cells of the Anuran Tadpole Tail : Its Isolation and Characterization in Vitro(Cell Biology)

概要

論文の詳細を見る
A method to isolate and maintain epidermal cells from the anuran tadpole has been developed in order to study hormonal effects on the growth and maintenance of the cells in vitro. Epidermal cells were released from bullfrog tadpole tails by treating with 2.5 mM EDTA and then with 0.25% trypsin and separated on a density gradient medium of Percoll. More than 98% of the cells thus prepared were epidermal cells. Viability of the isolated cells was around 90%. Attachment of the cells to plastic dishes was optimized by selecting RPMI-1640 as the culture medium and by coating dishes with fibronectin. Proliferation and maintenance of the cells were dependent upon the hormonal environment. The cells proliferated 2- to 3-fold within a few days and thereafter they began to leave dishes in a medium with normal serum. Cell attachment and proliferation were suppressed in a medium containing the serum deprived of thyroid and steroid hormones. Longevity of the cell, however, increased in this medium. Supplementation of the hormone-deprived medium with triiodothyronine and steroid hormones (5α-dihydrotestosterone, β-estradiol and hydrocortisone) changed the character of cells comparable to that found in a medium with normal serum. These hormonal dependencies of tadpole tail cells seem to reflect their character of in vivo status.
社団法人日本動物学会の論文
1985-04-15