Involvement of Calcium, lnositol-1, 4, 5 Trisphosphate and Diacylglycerol in the Prothoracicotropic Hormone-Stimulated Ecdysteroid Synthesis and Secretion in the Prothoracic Glands of Bombyx mori(Endocrinology)
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概要
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The objective of this study was to determine which intracellular second messenger systems are activated by prothoracicotropic hormone in the prothoracic glands (PGs) of Bombyx mori. Recombinant prothoracicotropic hormone (rPTTH) could stimulate ecdysteroid synthesis and secretion from day 6 PGs of the 5th instar of Bombyx mori within 30 min of in vitro incubation. However, rPTTH did not stimulate any increases in the glandular content of inositol 1, 4, 5-trisphosphate and cAMP during this short incubation period. Extracellular Ca^<2+> influenced the basal and rPTTH-stimulated ecdysteroid synthesis and release in a dose-dependent manner. The L-type Ca^<2+> channel antagonist, nitrendipine, inhibited the rPTTH-stimulated ecdysteroid synthesis and secretion (IC_<50>〜28μM). The phospholipase C inhibitor, 2-nitro-4-carboxyphenyl-N, N-diphenylcarbamate, inhibited the rPTTH-stimulated ecdysteroid synthesis (IC_<50>〜19μM). The protein kinase C inhibitor, chelerythrine chloride, inhibited the rPTTH-stimulated ecdysteroid synthesis (IC_<50>〜14μM). The protein kinase C activator, phorbol-12-myristate 13-acetate (PMA), could stimulate basal ecdysteroid synthesis and secretion (EC_<50>〜1μM) and its inactive α-isomer (4 α-PMA) was ineffective. The combined results suggest that the PTTH-stimulated ecdysteroid synthesis and release in the PGs of Bombyx is dependent on extracellular Ca^<2+> and the bifurcating second messenger signalling cascade of inositol 1, 4, 5-trisphosphate and diacylglycerol.
- 社団法人日本動物学会の論文
- 2001-12-10
著者
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FUGO Hajime
Department of Biological Production, Tokyo University of Agriculture and Technology
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Fugo Hajime
Department Of Biological Production Faculty Of Agriculture Tokyo University Of Agriculture And Techn
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Dedos Skarlatos
Institute of Biology, National Center for Scientific Research "Demokritos"
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Dedos Skarlatos
Institute Of Biology National Center For Scientific Research "demokritos
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Dedos Skarlatos
Institute Of Biology N.c.s.r. "demokritos
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