Shrinkage and Expansion of Blastocoel affect the Degree of Invagination in Sea Urchin Embryos(Developmental Biology)

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By immersing sea urchin embryos in seawater containing various concentrations of sucrose, we found that the level of osmotic pressure of blastocoel changed during gastrulation ; the level was low around the onset of gastrulation and higher during secondary invagination. To learn how such changes in osmotic pressure related to invagination processes, embryos were shrunken or expanded by means of sucrose treatments, and the degree of invagination was monitored using Nile blue staining. The method elucidated that the cells invaginated during primary invagination occupied only top one third of archenteron at the end of secondary invagination, and that the cells constituting intestine and the posterior half of stomach were recruited into the archenteron after secondary invagination. When embryos were expanded, the degree of invagination was remarkably decreased, indicating that a higher level of expanding force hindered the fulfillment of invagination. On the other hand, shrinkage of blastocoel during secondary invagination increased the degree of invagination. Cell tracing experiments showed that the change in the degree of invagination estimated with Nile blue staining was accompanied with the change in the number of cells incorporated into the archenteron. When embryos were expanded, only the descendants of veg2 constituted the gut rudiment, while a considerable amount of the vegl descendants were recruited into archenteron when embryos were shrunken. Obtained results suggest that change in the level of osmotic pressure of blastocoel would be necessary for the progress of gastrulation.
社団法人日本動物学会の論文
2001-11-01

Shrinkage and Expansion of Blastocoel affect the Degree of Invagination in Sea Urchin Embryos(Developmental Biology)

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