Induction of Bent Cartilaginous Skeletons and Undulating Notochord in Flounder Embryos by Disulfiram and α, α'-Dipyridyl(Developmental Biology)
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概要
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Disulfiram causes undulation of the notochord and bending of pharyngeal cartilages in fish embryos. Using flounder embryos, this study aimed to elucidate which process of pharyngeal arch development was affected by the drug. Since disulfiram is known to block the synthesis of retinoic acid (RA) in vivo, we first examined whether the drug suppresses the expression of Hoxd-4 and shh, RA responsive genes related with the pharyngeal arch development. Disulfiram at a concentration which induces undulation of the notochord and bending of cartilage elements did not affect the expression of these genes. On the other hand, similar phenotypes of anomalies were found to be reproduced both in the notochord and pharyngeal cartilages by α, α'-dipyridyl which reduces the mechanical stability of collagen. Thus, we suppose that disulfiram causes anomalies by decreasing the mechanical stability of collagen and not by suppressing the expression of RA-responsive genes. Since disulfiram blocks ascorbate dehydrogenase, it is our hypothesis that this drug inhibits the maturation of collagen by affecting ascorbic acid metabolism.
- 社団法人日本動物学会の論文
- 2001-04-10
著者
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Suzuki Tohru
Metabolism Section, National Research Institute of Aquaculture, Fisheries Agency
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Kurokawa Tadahide
Metabolism Section National Research Institute Of Aquaculture Fishery Agency
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Srivastava Anand
Metabolism Section National Research Institute Of Aquaculture Fishery Agency:(present Address)uc303
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Suzuki Tohru
Metabolism Section National Research Institute Of Aquaculture Fishery Agency
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Suzuki Tohru
Metabolism Section Fish Nutrition Division National Research Institute Of Aquaculture
関連論文
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- Activity and Expression of Glutamate Oxaloacetate Transaminase during the Reproductive Cycle of a Fresh Water Fish Labeo rohita
- Retinoic acid given at late embryonic stage depresses sonic hedgehog and Hoxd-4 expression in the pharyngeal area and induces skeletal malformation in flounder (Paralichthys olivaceus) embryos
- Hoxd-4 Expression during Pharyngeal Arch Development in Flounder(Paralichthys olivaceus)Embryos and Effects of Retinoic Acid on Expression
- Functional Analyses of FGF during Pharyngeal Cartilage Development in Flounder (Paralichthys olivaceus) Embryo
- Induction of Bent Cartilaginous Skeletons and Undulating Notochord in Flounder Embryos by Disulfiram and α, α'-Dipyridyl(Developmental Biology)