Expression of Gill Vacuolar-Type H^+-ATPase B Subunit, and Na^+, K^+-ATPase α_1 and β_1 Subunit Messenger RNAs in Smolting Salmo salar(Physiology)

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概要

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• Changes in gill vacuolar-type HT-ATPase B subunit, and Na^+,K^+-ATPase α and β subunit mRNA expression were examined during the course of smoltification in Salmo salar. We cloned and sequenced cDNA fragments of S. salar gill i) vacuolar-type H^+-ATPase (V-H^+-ATPase) B subunit, ii) Na^+,K^+-ATPase α_1 subunit, and iii) Na^+,K^+-ATPase β_1 subunit, and used these as Northern blotting probes. During smoltification, the salmon showed a typical increase in gill Na^+,K^+-ATPase activity and improved hypo-osmoregulatory ability as judged by their ability to regulate plasma [Cl^-] in a 24-hr seawater challenge test (35 ppt). Gill Na^+,K^+-ATPase α_1 and β_1 subunit mRNA levels were regulated at a constant ratio during smoltification. Both transcripts were elevated during the build-up of gill Na^+,K^+-ATPase activity, underlining the importance of increased mRNA levels for increased enzyme activity. Gill V-H^+-ATPase B subunit mRNA levels were high during the early phase of smoltification. These results support our hypothesis that gill V-H^+-ATPase expression may be elevated during the early stages of smoltification in order to counter the effects of increased ionic efflux when in FW. The peak smolt stage was, however, characterized by simultaneously elevated gill Na^+,K^+-ATPase expression and low V-H^+-ATPase expression, and possibly ensures the complete transformation of the gill into a hypo-osmoregulatory organ and hence the development of optimal SW-tolerance of the smolt.

• 社団法人日本動物学会の論文
• 2001-04-10

著者

• Cramb Gordon

  School Of Biomedical Sciences Bute Medical Buildings University Of St. Andrews

• Seidelin Michel

  Institute of Biology, University of Southern Denmark-Main Campus

• Madsen Steffen

  Institute of Biology, University of Southern Denmark-Main Campus

• Cutler Christopher

  School of Biomedical Sciences, Bute Medical Buildings, University of St. Andrews

• Madsen Steffen

  Institute Of Biology University Of Southern Denmark-main Campus : Odense University

• Madsen Steffen

  Institute Of Biology Odense University

• Cutler Christopher

  School Of Biomedical Sciences Bute Medical Buildings University Of St. Andrews

• Seidelin Michel

  Institute Of Biology University Of Southern Denmark-main Campus : Odense University:(present Address

• Seidelin Michel

  Institute Of Biology University Of Southern Denmark-main Campus : Odense University:(present Address

関連論文

• Expression of Gill Vacuolar-Type H^+-ATPase B Subunit, and Na^+, K^+-ATPase α_1 and β_1 Subunit Messenger RNAs in Smolting Salmo salar(Physiology)
• Prolactin Antagonizes the Seawater-Adaptive Effect of Cortisol and Growth Hormone in Anadromous Brown Trout (Salmo trutta)
• Vacuolar-Type H^+-ATPase and Na^+, K^+-ATPase Expression in Gills of Atlantic Salmon (Salmo salar) during Isolated and Combined Exposure to Hyperoxia and Hypercapnia in Fresh Water(Physiology)

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