Identification of a Homolog of Actin-Binding Protein, ABP-280, Localized at Epithelial Cell-Cell Boundaries in Hydra(Cell Biology)

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概要

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• ABP-280 cross-links actin filaments and connects membrane glycoproteins and actin filaments in mammalian blood cells. We isolated cDNA clones for ABP-280 from hydra by screening a cDNA expression library with an antiserum against a cell membrane-enriched crude fraction. The amino acid sequences deduced from the cDNA clones showed significant similarity to human ABP-280, including intramolecular repetition of a unique sequence motif, suggesting similar tertiary structure and molecular activity. Monospecific antibodies were purified from the antiserum, by affinity to fusion proteins produced from the cDNA clones, and used for immunohistochemistry. Specific staining was detected at epithelial cell-cell boundaries in the hydra. The signals were restricted to the subapico-lateral regions corresponding to the locations of septate junctions. These results suggest that the identified molecule is a possible component of the septate junction, and presumably connects membrane cell adhesion molecules to actin filaments in the epithelial cells of hydra. This is the first report describing the subcellular distribution of ABP-280-related molecules in epithelial cells.

• 社団法人日本動物学会の論文
• 1999-06-15

著者

• Koizumi O

  Environmental Science Fukuoka Women's University

• Kobayakawa Yoshitaka

  Faculty of Science, Kyushu University

• Hatta Masayuki

  National Institute of Genetics

• Sakaguchi Masahiko

  Faculty of Education, Shinshu University

• Kishimoto Yasuyuki

  National Institute of Genetics

• Koizumi Osamu

  Fukuoka Women's University

• Hatta M

  Ochanomizu Univ. Tokyo Jpn

• Hatta Masayuki

  Marine And Coastal Biology Center Ochanomizu University

• Kishimoto Yasuyuki

  National Institute Of Genetics:(present Address)max-planck Institute For Developmental Biology

• Sakaguchi M

  Berlin Free Univ. Berlin Deu

• Kobayakawa Y

  Faculty Of Science Kyushu University

• Kobayakawa Yoshitaka

  Faculty Of Science Kyushu University

関連論文

• COMBINATION OF HYDRAPEPTIDE PROJECT AND HYDRAEST PROJECT FOR SYSTEMATIC IDENTIFICATION OF NOVEL NEUROPEPTIDES(Physiology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• TEMPORAL AND SPATIAL DEVELOPMENT OF NERVOUS SYSTEM DURING EMBRYOGENESIS OF NEMATOSTELLA VECTENSIS (ANTHOZOA, CNIDARIA)(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• Identification of a Homolog of Actin-Binding Protein, ABP-280, Localized at Epithelial Cell-Cell Boundaries in Hydra(Cell Biology)
• Structure-Activity Relation of LWamide Peptides Synthesized with a Multipeptide Synthesizer
• REGULATION OF METAMORPHOSIS OF THE CORAL ACROPORA IN RESPONSE TO ENVIRONMENTAL CUES(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• APPEARANCE OF PREDATOR PLANULAE(Behavior Biology and Ecology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• Phylogenetic Relationships in the Coral Family Acroporidae, Reassessed by Inference from Mitochondrial Genes(Taxonomy)
• Reticulate evolution of corals
• EARLY DEVELOPMENT OF THE REEF-BUILDING CORAL ACROPORA(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
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