Cloning and Characterization of cDNAs Encoding Trehalase from Post-Dormant Embryos of the Brine Shrimp, Artemia franciscana(Developmental Biology)
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概要
- 論文の詳細を見る
To investigate post-dormant regulation of trehalose metabolism in the brine shrimp, we cloned and characterized two trehalase cDNAs from embryos of Artemia franciscana using a PCR probe corresponding to a highly conserved region among trehalases. The cDNAs consisted of 2496 and 2485 nucleotides, and had almost the same open reading frame encoding 703 amino acids which showed 46.6-42.6% similarities to trehalases of animals. The calculated molecular mass of the trehalase was 79,995 Da. The deduced sequence had a cleavable signal peptide, a cell adhesion motif, four potential N-glycosylation sites, trehalase signatures and a unique, long carboxyl terminal polypeptide containing a predicted transmembrane region and a potential cAMP-dependent phosphorylation site. Phylogenetic analyses showed a large divergence among trehalases of arthropods. Northern blots revealed the presence of two mRNAs. One of them, a 2.6 kb mRNA, was abundant in the dormant cysts and prenauplii. The other 5.0 kb mRNA was newly synthesized during post-dormant development. Possible mechanisms of trehalase regulation are presented on the basis of the results shown by the Northern blots and developmental changes of trehalase activity.
- 社団法人日本動物学会の論文
- 1999-04-15
著者
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Tanaka Shin
Biology, Department of Medical Technology, School of Health Sciences, University of Occupational and
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Nambu Fumiko
Biology, Department of Medical Technology, School of Health Sciences, University of Occupational and
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Nambu Ziro
Biology, Department of Medical Technology, School of Health Sciences, University of Occupational and
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Nambu Fumiko
Biology, Department of Medical Technology, School of Health Sciences, University of Occupational and Environmental Heaalth
関連論文
- mRNA PROFILING OF DORMANT CYSTS AND POST-DORMANT EMBRYOS OF BRINE SHRIMP, ARTEMIA FRANCISCANA(Genetics,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- PARTIAL BIVOLTINE IN EACH BROOD OF ARTEMIA DORMANT CYSTS(Physiology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- PROPERTIES OF INBRED STRAINS OF ARTEMIA FRANCISCANA(Behavior Biology and Ecology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- ALTERNATIVE SPLICING OF mRNA DERIVED FROM ARTEMIA TREHALASE GENE(Genetics,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- CHARACTERIZATION OF GENETIC VARIATION AMONG INBRED STRAINS OF BRINE SHRIMP (ARTEMIA FRANCISCANA) USING RAPD MARKERS(Genetics,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- TERMINATION OF EMBRYONIC DIAPAUSE IN ARTEMIA FRANCISCANA(Physiology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- CLONING OF THE TREHALASE GENE FROM THE BRINE SHRIMP, ARTEMIA FRANCISCANA(Genetics)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- ESTABLISHMENT OF INBRED STRAINS OF ARTEMIA FRANCISCANA(Taxonomy and Systematics & Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- EFFECTS OF PHOTOPERIOD AND TEMPERATURE ON THE BREEDING PATTERN OF THE BRINE SHRIMP, Artemia franciscana(Physiology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Whole mount immunological detection of SPARC protein in crustacean Artemia nauplii(Cell Biology and Morphology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- TRIAL TO ESTABLISH INBRED STRAINS OF Artemia franciscana(Taxonomy and Systematics)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- ANTI-BOVINE SPARC MONOCLONAL ANTIBODY REACTIVE PROTEIN IN DEVELOPING ARTEMIA(Biochemistry)(Proceedings of the Seventieth Annual Meeting of the Zoological Society of Japan)
- Cloning and Characterization of cDNAs Encoding Trehalase from Post-Dormant Embryos of the Brine Shrimp, Artemia franciscana(Developmental Biology)
- Characterization of SPARC(BP-40, osteonectin) cDNA from developing Artemia franciscana(Developmental Biology)(Proceedings of the Sixty-Ninth Annual Meeting of the Zoological Society of Japan)
- Purification and Characterization of Trehalase from Artemia Embryos and Larvae