Lactate Dehydrogenase of the Horseshoe Crabs and Their Hybrids(Biochemistry)

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概要

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• The lactate dehydrogenase (LDH) of the horseshoe crabs and their hybrids was analyzed with specific enzyme staining on starch gels. The LDH from hepatopancreas tissues of Tachypleus tridentatus and Carcinoscorpius rotundicauda consisted of 3 isozymes and the Tachypleus gigas LDH from hepatopancreas tissue occurred in 1, 3, or 6 enzymic bands. The American horseshoe crab, Limulus polyphemus, had the 2 LDH triads. Each embryonic LDH of 3 Asian horseshoe crabs showed only one electrophoretic band and the LDH of interspecific hybrid embryos occurred in 3 electrophoretic bands, suggesting that each embryonic LDH molecule of the 3 Asian species was a homodimer and that each LDH of the hybrid embryos was composed of a paternal homodimer, a maternal homodimer, and a hybrid heterodimer. Electrophoretic patterns of the LDH in the Asian horseshoe crabs and their hybrids support the dimer model that the LDH triad is genetically controlled by 2 loci and that individuals with the common phenotype (3 dimer bands) are homozygous at both loci. The T. gigas♀ × C. rotundicauda♂ eggs were grown into the first-instar larvae for the first time since the first experimental hybridization had been carried out in 1977. The LDH of this unusual hybrid also consisted of a paternal homodimer, a maternal homodimer, and a hybrid heterodimer.

• 社団法人日本動物学会の論文

著者

• Sekiguchi Koichi

  Institute Of Biological Sciences University Of Tsukuba

• Sekiguchi Koichi

  Institute Of Biological Sciences The University Of Tsukuba

• Sugita Hiroaki

  Institute Of Biological Science University Of Tsukuba

関連論文

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• Identification of Orthologous Hemocyanin Subunits in Scorpions : The First Step to Produce a Phylogenetic Tree Using Amino Acid Sequence Data
• Amplexus and Mating Behavior in the Japanese Horseshoe Crab, Tachypleus tridentatus
• Lactate Dehydrogenase of the Horseshoe Crabs and Their Hybrids(Biochemistry)
• Acid Mucopolysaccharide in Embryos of the Horseshoe Crab, Tachypleus tridentatus : Chelicerata, Arthropoda
• Swelling Mechanism of the Embryo of the Japanese Horseshoe Crab, Tachypleus tridentatus
• A Case of Orthologous Sequences of Hemocyanin Subunits for an Evolutionary Study of Horseshoe Crabs : Amino Acid Sequence Comparison of Immunologically Identical Subunits of Carcinoscorpius rotundicauda and Tachypleus tridentatus
• N-Terminal Amino Acid Sequence Comparison of Asian Horseshoe Crab Hemocyanins: Immunologically Identical Hemocyanin Subunits Are Orthologous in Asian Horseshoe Crabs

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