Functions of a Maternal Gene are Required for the Synchronous Nuclear Division during Early Development of Drosophila melanogaster. Analyses on a ts-Mutant(Cell Biology)

概要

論文の詳細を見る
Temperature-shift analyses were performed on a maternal-effect, temperaturesensitive (25℃ as restrictive and 1 8℃ as permissive temperature) mutant of' Drosophila melanogaster, gs(1)N20. The results from the temperature-shift experiments under four different plans characterize the temperature sensitivity of this mutant as the following. (1) Temperature-sensitive period (TSP) of this mutant is in the pre-vitellogenic and germarium stages of oogenesis. (2) Exposure of ovarian eggs to 18℃ either during all pre-vitellogenic stages (stage 2-7, or 4 days at 18℃), or during all germarium stages from the very beginning of ovary organization is sufficient for the expression of wild-type phenotype, even if the eggs are exposed to 25℃ during the rest of the stages of oogenesis. (3) Exposure of ovarian eggs to 18℃ for 10 days during their germarium stages is insufficient for wildtype expression. This mutant has been screened for the grandchildless phenotype, and the TSP was determined for this phenotype. However, recent understanding that the grandchildless phenotype is ascribed to the disorder in synchronous nuclear division in the early embryogenesis, supports the idea that the reaction generated by gs(1)N26^+ gene may be involved in a mechanism to organize a condition for the synchronous intravitelline mitosis. The present work has revealed that this mechanism works during very early stages of oogenesis.
社団法人日本動物学会の論文