Partial Biochemical Characterization of Humoral Factors Involved in the Nonfusion Reaction of a Botryllid Ascidian, Botrylloides simodensis

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概要

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• By means of an in vivo bioassay technique based on the micro-injection of blood plasma, it has been demonstrated that the blood plasma of Botrylloides simodensis has a specific activity corresponding to the colony specificity or allogeneic histoincompatibility. When intact blood plasma is exposed to various physico-chemical conditions, the specific activity is easily replaced by a nonspecific activity. The specific and nonspecific activities have some characteristics in common, both being resistant to dialysis and heat-labile and dependent on bivalent cations. These facts suggest that both activities are attributable to the same factor in the blood plasma. Furthermore, the nonspecific activity appears in large molecule fractions either by ammonium sulfate fractionation or by Sephadex G75 gel filtration. The activity is not affected by trypsin, protease or neuraminidase.

• 社団法人日本動物学会の論文

著者

• Saito Yasunori

  Shimoda Marine Research Center, University of Tsukuba

• WATANABE Hiroshi

  Shimoda Marine Research Center,University of Tsukuba

• Saito Yasunori

  Shimoda Marine Research Center Univ. Of Tsukuba

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