温度衝撃によって生じる両生類の異常胚

概要

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Embryos of Bufo valgaris, Rana japonica and Rana nigromaculata from the cleavage stage to the tail-bud stage were exposed to high temperatures, 34-40℃, for 15 and 20 minutes, and then kept in tap water at 18-20℃. The optimum temperature for causing abnormalities increased with the progress of development. The highest frequency of abnormalities was attained when the embryos were treated at the early gastrula stage. The obtained abnormalities were clanified into neurula with yolk plug, spina bifida, kyphosis and tail-bent embryo. Generally, the epidermis was wrinkled, and neural differentiation was affected in various ways. Suppression of morphogenetic movements inducing ablity and differentiation of the organizer area was also remakable. Within the endoderm abnormal cell groups appeared, in which yolk platelets indicated various steps of disintegration. These cell groups were intensively stained by hematoxylin after Bouin fixation.
社団法人日本動物学会の論文
1962-04-15