マウス卵巣内の異常成熟卵子に及ぼすP.M.S.の効果についての細胞学的研究
スポンサーリンク
概要
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In a previous paper, Nakamura ('57) reported the cytological features of abnormal mature ova which could not undergo ovulation in the normal sexual cycle of mice. It seems probable that the disintigration takes place in both the egg body and granulosa cells under the influence of pituitary gonadotrophic hormones on the atretic follicle. In a previous study, Nakamura ('59) reported the effect of sex stimulating hormones from the anterior pituitary gland and placenta on abnormal mature ova in mice. The present study was undertaken to investigate the effect of pregnant mare serum of horses on abnormal ova during the sexual cycle of mice. The material used was unmated mature female mice (Swiss albinos) at 70-120 days of age. Starting 5 days before the estrus stage, individual mice received injections of sex stimulating hormones from the anterior pituitary and placenta, 2 R.U. per day for 3 successive days and gain an injection of pregnant mare serum of horses, 50 I.U. Twenty-four after the above treatment, ovaries were fixed and prepared for microscopical study. Microscopical observations revealed that abnormal mature ova preceding further than the first maturation stage are very few in number in P.M.S. treated mice than in untreated mice. Twenty-five treated mice showed in their ovaries 308 normal ova, 46 abnormal ova and 32 polyovular ova in total. Twenty untreated mice showed in their ovaries 157 normal, 85 abnormal and 80 polyovular ova. Twenty-five mice treated with anterior pituitary and placenta hormones showed in their ovaries 202 normal, 45 abnormal and 48 polyovular ova in total. Evidence presented indicates the remarkable reduction in number of the abnormal ova in P.M.S. treated mice. Particulary, the decrease in number of abnormal ova showing an immature condition of granulosa cells is remarkable.
- 社団法人日本動物学会の論文
- 1959-10-15