組織化学的に見たニシン卵の初期発生

概要

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The early development of the herring eggs was studied from the histochemical viewpoint. The following substances were detected: Iron (Turnbull blue reaction or Berlin blue reaction. For unmasking, Macallum's technique), polysaccharides (Bauer's chromic acid-Schiff reaction or Hotchkiss periodic acid-Schiff reaction), SH-groups (Chevremont and Frederic's ferric-ferricyanide reaction in which the sections were pre-treated with 10% KCN for 5 minutes), lipid (Ciaccio's technique) and nucleic acid (for DNA, Feulgen's reaction; for RNA, staining with pyronin and digestion with RNase). The results are as follows: 1.The eggs at various developmental stages do not show the reaction for iron by the methods used. 2.The egg membrane and the cortical alveoli contain polysaccharides, protein and disulfide bridges. 3.The substances detected in the present study distribute nearly uniformly in the blastodisc of the egg and no regional variation in the concentration of these substances is observed. 4.Periblast plays a role in the absorption of the yolk after gastrulation.
社団法人日本動物学会の論文
1957-07-15