ゾウリムシParamecium caudatumの無菌培養
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概要
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Being in pursuit of establishing the sterile culture of Paramecium coudatum, we have carried out the present experiment, which revealed some interesting problems, though requiring further investigation. 1.The material used in this study is P. caudatum. 2.The sterile culture medium is in the main the same as that proposed by Johnson (1952) for P. aurelia and P. multimicronucleatum. It is composed mainly of 1% solution of polypeptone (10 parts), autoclaved pressed yeast juice (1 part) and hydrolyzed nucleic acid (100γ/ml of DNA or RNA). A sterile clone of P. caudatum was successively renewed 6 times at every 5 or 6 days, the fission rate being 0.4 to 0.6 per day at 22℃ without showing any sign of degeneration. Of the three components, the first and the third can be replaced respectively with the enzymatically hydrolyzed milkcasein and the mixture of mononucleotides containing adenylic and guanylic acids. But the second has no substitute available. 3.The fission rate in our sterile case is much lower than that of the two-membered culture. In our case, however, tha sterile animals took somewhat swollen and eliptical form. This may be apparantly and in all probability partly due to differences in the constitutions of the culture media. So long as our culture were maintained, both conjugation and autogamy could not be induce at all. 4.As show in the preliminary experiments, P. caudatum can put up considerably with the action of penicillin. For instance, P. caudatum can be kept alive for about 24 hours in the solution of 10000 units/ml of penicillin. Therefore, it was possible to prepare materials with no external contaminant by means of effective application of penicillin, but it seems extremely difficult but not absolutely impossible at the present state of our technique to get material free from internal contaminant.
- 社団法人日本動物学会の論文
- 1956-12-15