トノサマガエル半数体の實驗的作製

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概要

• 論文の詳細を見る

• Experiment were made to find out the most reliable and easy method of producing gynogenetic haploids in the frog. For this purpose, Rana nigromaculata eggs were inseminated with sperm treated with toluidine-blue or trypaflavine. (1) Good results were obtained by treatment of sperm with about 0.002% trypaflavine or 0.0002% toluidine-blue solution for 30 minutes. (2) The action of trypaflavine seemed to be strongly influenced by the temperature. Trypaflavine produced no haploids at about 25℃, in contrast to toluidine-blue which always produced a great many haploids at various temperatures. (3) By insemination of R. nigromaculata eggs with R. japonica or R. rugosa sperm treated with toluidine-blue, many haploid larvae were readily produced. (4) Among larvae with abnormal appearances, various kinds of mosaics were found by cytological observations.

• 社団法人日本動物学会の論文
• 1954-05-15

著者

• 宮田 澄男

  兵庫農科大・生

• 宮田 澄男

  神戸大学教養部生物学教室

関連論文

• 綜合討論(形態学・遺伝学)
• 精子の色素處理によるトノサマガエル半數體幼生の作製(實驗形態・發生)
• 半数性ダルマガエルの発生に伴う可溶性タンパク質の電気泳動像の変化(発生)
• 半数性カエルの幼胚の生活力と核酸合成との関係(発生)
• 半数性カエルの初期発生に対する比較的低温の阻害作用(発生(受精・卵形成))
• 採餌し変態したトノサマガエル半数体幼生について(発生学・実験形態学)
• トノサマガエル半数体の實驗的作製
• 変態を完了した半数性トノサマガエルについて
• 家兎心電図に及ぼす体位及び前肢位置の影響
• トノサマガエル半数体幼生の酸素消費量

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