イモリの磨碎肝臟組織による發情ホルモン物質の破壊

概要

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Different doses (50-200 i.n.) of the commercial estrogen (ovahormone Takeda) were added to lg of pulp of liver of the newt, Triturus pyrrhogaster. After allowing to stand for 24 hours at room temperature, the mixture was extracted for 60 hours with acetone and then for 50 hours with alcohol in a Soxhlet. The extracts, combined after evaporation of the solvents, were taken in 0.8-1 cc of olive oil or whale oil and injected into spayed female rats. It was found that when 80 i.u. or more of the estrogen had been added to the liver pulp the doses sufficient to induce estrus in the rats sometimes escaped inactivation but when 60 i.u. or less had been added effective doses never remained. In the control experiments with lg of stomach tissue plus 50 i.u. of the estrogen the extracts were always estrogenic.
社団法人日本動物学会の論文
1950-04-15