家蠶に於けるプテリンの存在とその遺傳生化學的考察(豫報)

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The normal (p^+) and oily translucent (od) body colour of silkworms respectively has been reported to be sought in the quantitative difference of uric acid or its salts. Besides these strains, thre is another yellow coloured one (lem), containing yellow pigments in its hypodermis. And again, it has been well known that the body colour characteristic to each strain, which having genetically an intimate relation with melanin granules, uric acid or its salts, and further lactflavin contained in Malpighian vessels, has taken specific behavior in its appearance (Kikkawa '43 Aruga '44, '47), but the chemical nature of which remains undissolved. The desire to clarify its chemical nature of colour in hypodermis and Malpighian vessels motivated the experiments here. Materials tested were tabulated in table 1. The procedure adopted being as follows: by the method of paper chromatography (solvent: butyl alcohol-acetic mixture or butyl alcohol alone), the colour of fluorescence of substances, which were located on the strip separately in response to their character, was examined in ultra-violet light. Substances were found in extracts of 0.5 N ammonium hydroxide or distilled water. As indicators, pterins in the white (Pieris rapae L.) and yellow (Colias hiale poligraphus Motschulsky and Eurema hecabe L.) butterfly wings, synthetized xanthopterin and lactflavin (Hoffmann-Roche) were employd. Results obtained are as follows. (table 2 and 3, Fig. 1) In the hypodermis, pterins were found, but not lactflavin. No difference was detected in fluorescence and its location on the strip among the normal oily strains and white butterfly wing, pale blue and purple colour being present, and also no difference between the yellow strain and yellow butterfly wing, greenish blue, purple, purplish blue and pale blue being present. In the former case, the fluorescence of pale blue was assumed to be leucopterin and in the latter case, that of greenish blue, was identified as xanthoptein. This identification was further ascertained by the change of pH value and treatment with acetic acid, strong sulphuric acid, sodium carbonate and sodium hydroxide. Although the pruple fluorescence was remarkably observed not only in the above extracts, but also in the black portion of silkworms and butteflies, it has been reported that this compound is obsucure in exact nature (Good & Johnson '49) or to be a decomposition product of xanthopterin (Schopf & Becker '36) But, from the state of appearance it is expected to be a new pterin. Next, in Malpighian vessels, no pterins were present, but lactflavin was found in great abundance in the normal strain, while scarcely found in the oily one. Further, red pigments, which were situated on the upper part near the stigma of "striped" (PS) and "zebra" (Ze) strains were examined without being ascertained to be pterin.
社団法人日本動物学会の論文
1949-12-15

家蠶に於けるプテリンの存在とその遺傳生化學的考察(豫報)

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