發生學的に見たるバフンウニの卵の細胞質構造
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概要
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The living egg of a sea urchin, Strongylocentrotus pulcherrimus (A. AGASSIZ), contains light orange pigment. The author found that the pigment was soluble in acetone, and that the acetone solution of the pigment looks dark red, when it is abserved through a blue filter, N. 49 WRATTEN tricolor filter. By putting the blue filter between the light source and the condensor of the microscope the localization of the pigment in the living egg become observable. With this method it was ascertained that the pigment of the egg is not diffusedly dissolved in the cytoplasm, but is found in the form of many fine granules. The pigment granules are mostly in the peripheral portion of the egg, i. e. in the cortical cytoplasm. And, it was possible to follow the behavior of the cortical cytoplasm by marking the pigment granules as the indicator of the cortical cytoplasm. The results of observations on the behavior of the cortical cytoplasm by using this new optical method were as follows: The cortical cytoplasm of the egg of the sea urchin is not carried inside the embryo by the protoplasmic flow in the course of normal cleavages. The relative position of the cortical cytoplasm at the surface of the egg is not changeable by the cleavage furrow in the normal development. The cell boundary between the blastomeres of the normal developing egg was newly produced in the course of cleavage. When the egg was cultured in Ca-free sea water, the pigmented cortical cytoplasm covers the whole surface of the blastomere in the early cleavage, and the new formation of the cell boundary between the blastomeres was not observed. And when the egg was cultured further in this solution, the normal embryo was not formed. in this case the cortical cytoplasm lost the superficial position in the embryo.
- 社団法人日本動物学会の論文
- 1936-03-15