Light Microscopic Visualization of Nuclear Architectures in Chick Embryonic Cells
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概要
- 論文の詳細を見る
A new method for light microscopic visualization of nuclear chromatin networks or loopings was described briefly in this report. Small tissue-pieces were squashed in 0.5% SDS solution immediately after having been suspended over 100% alcohol for a moment. The squashed preparation was fixed and stained with a glutaraldehyde/glyoxal(1:1) mixture containing methylgreen/pyronin. A nuclear preparation several times more expanded than an average size in situ nucleus was obtained enabling observation of gross chromatin networks under the light microscope. Unique loopings were also observed in preparations given the short alcohol treatment.
- 社団法人日本動物学会の論文
- 1983-09-25