Primary Tissue Culture of Cells in the Land Slug Incilaria bilineata

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概要

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• Primary tissue culture of the land slug, Incilaria bilineata was maintained for 2 or 3 months in a medium based on the concentrations of Na, K, Ca and free amino acids in slug hemolymph. No cell division was observed, but the cells survived. Thin medium layer culture which contained 1.0 ml of medium per 5 cm plastic Petri-dish was used. The small minced tissue pieces were explanted per dish. The medium was renewed every third day. This method enabled long term cultivation. The concentration of alanine in the medium increased during 4 days of cultivation to ca. 20 fold that of the incubated control level without cells. When slug hemolymph was added into the medium, it inhibited the migration of slug cells. However, the hemolymph heated at 56℃ for 30 min neither inhibited the migration nor induced cell division.

• 社団法人日本動物学会の論文
• 1980-09-25

著者

• Furuta Emiko

  Department Of Anatomy Dokkyo University School Of Medicine

• Manaka Ken-ichi

  Department Of Anatomy Dokkyo University School Of Medicine

• Shimozawa Atsumi

  Department of Anatomy, Dokkyo University School of Medicine

• Shimozawa Atsumi

  Department Of Anatomy Dokkyo University School Of Medicine

関連論文

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• Chronic Skin Allograft Rejection in Terrestrial Slugs(Immunology)
• PHAGOCYTOSIS OF HEMOCYTES FROM SCHISTOSOMA-RESISTANT AND SUSCEPTIBLE ONCOMELANIA NOSOPHORA AGAINST SCHISTOSOMA JAPONICUM SPOROCYSTS(Physiology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• Primary Culture of cells from the Foot and Mantle of the Slug, Incilaria frukstorferi Collinge
• Primary Tissue Culture of Cells in the Land Slug Incilaria bilineata
• Chemotaxis in the Freshwater Planarian, Dugesia japonica japonica(Behavior Biology and Ecology)

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