Spontaneous Cytotoxic Earthworm Leukocytes Kill K562 Tumor Cells

概要

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Earthworm coelomocytes may act as effector cells which destroy targets in vitro. In a ^<51>Cr release assay, Lumbricus coelomocyte effectors showed lytic activities of 3-14% against K562 human tumor cells when incubated 1-4 hr at 23℃ or 37℃. Cytotoxicity was correlated with effector: target ratio. However, targets were not killed by incubating them in cell-free, 0.2 μm filtered coelomic fluid. The supernatant from coelomocytes cultured alone failed to kill K562 targets but coelomocyte lysates were toxic to target cells in a concentration-dependent manner. Coelomocytes were examined using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). When effectors and targets were examined under TEM, we found close apposition of effector granulocytic coelomocytes and target cell membranes but not with coelomocytes nor eleocytes at up to 15 min incubation. By SEM, effector cells appeared not only to be in close contact with targets, but instances of target lysis were observed. These results suggest that effector cell/target cell contact is essential for cytotoxicity to occur.
社団法人日本動物学会の論文
1995-08-15