Androgen-induced Differentiation of the Fibrocartilage of Os Penis Cultured in vitro

概要

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Primordia of the fibrocartilage of os penis of the rat were cultured in vitro in the presence of testosterone, and the effects of testosterone on the expression of type I collagen, type II collagen, vimentin, the activity of alkaline phosphatase (ALP), and Alcian blue-stainable extracellular matrix (ECM) were examined histochemically. Explants cultured in the presence of testosterone formed fibrocartilage while control explants cultured without testosterone formed fibroblastic tissue. Type II collagen, ALP, and Alcian blue stainable ECM were detected only in the explants that had been cultured with testosterone. Calcification of the fibrocartilage was observed in explants that had been cultured for 21days. By contrast, levels of type I collagen and vimentin increased in explants cultured with or without testosterone. These results demonstrate that testosterone induces terminal differentiation of the fibrocartilage while some histological markers of fibrocartilage can be expressed in the absence of testosterone.
社団法人日本動物学会の論文
1994-12-15