Effects of Hypophysectomy and Replacement Therapy with Bovine Growth Hormone and Triiodothyronine on the in Vitro Uptake of Calcium and Methionine by Scales in the Goldfish, Carassius auratus

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Using isolated scales, the involvement of bovine growth hormone (bGH) and triiodothyronine (T_3) in scale formation was examined in the hypophysectomized goldfish, Carassius auratus. The in vitro uptake of calcium and ^<35>S-methionine by scales was used as indices of their calcification and matrix formation, respectively. Hypophysectomy consistently decreased the uptake of these substances with time up to 4 weeks after the operation. The direct addition of bGH or T_3 to the incubation medium had no recovery effect. However, the decreased uptake was restored in the incubation medium with the serum not of hypophysectomized but of sham-operated fish. The acid extract of the hepatopancreas was also effective for stimulating the uptake. The muscle, kidney, and spleen were ineffective for the extract-stimulated uptake. The hepatopancreas-stimulated uptake was also confirmed by the addition of the preincubation media in which the minced hepatopancreas was incubated with various concentrations of bGH or T_3 for 24 hr. These results suggest that bGH and T_3 stimulate scale calcification and matrix formation not via direct but via indirect sequences. A factor(s) secreted from the hepatopancreas in response to bGH and T_3 was considered to exert direct stimulative effects on scale formation.
社団法人日本動物学会の論文
1994-08-15