Localization and Purification of Serum Albumin in the Testis of Xenopus laevis
スポンサーリンク
概要
- 論文の詳細を見る
The distribution of serum albumin is of interest in the Xenopus (X.) laevis testis, since albumin is probably a major protein that binds testosterone (T) in the plasma and interstitial fluid. This study was undertaken to determine the localization and purification of serum albumin in the X. laevis testis. The interstitial tissue and spermatogonia immunoreacted strongly with a sheep antiserum raised against X. laevis albumin. A weak staining was also seen in spermatocytes and early spermatids, but there was no staining in Sertoli cells. In order to clarify whether serum albumin was really localized on the surface of testicular cells in the X. laevis testis, a membrane-rich fraction was prepared from testes and extracted with 0. 6M KCl. The KCl extract was then subjected to gel filtration, ammonium sulfate precipitation and high-performance liquid chromatography (HPLC). A protein with Mr = 74 kD was obtained by this procedure and its NH_2-terminal amino acid sequence was determined. The sequence of the first 19 amino acids was DTDADXXKXIADVYTALTE, suggesting that this protein was identical to serum albumin (Mr=74kD). When the membrane fraction of blood cells in this animal was handled in the same manner, no appreciable amount of albumin was detected . These results suggest that the 74 kD serum albumin, possibly associated with bound T, may play an important role in the differentiation of germ cells during spermatogenesis of X. laevis testis.
- 社団法人日本動物学会の論文
- 1994-04-15
著者
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Nakamura M
Laboratory For Amphibian Biology Faculty Of Science Hiroshima University
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Nakamura Masahisa
Laboratory for Amphibian Biology, Faculty of Science, Hiroshima University
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Yamanobe Tomoyo
Central Laboratory of Analytical Biochemistry, School of Medicine, Teikyo University
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Takase Minoru
Laboratory for Amphibian Biology, Faculty of Science, Hiroshima University
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Takase M
Hiroshima Univ. Hiroshima Jpn
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Takase Minoru
Laboratory For Amphibian Biology Faculty Of Science Hiroshima University
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Yamanobe T
Teikyo Univ. Tokyo Jpn
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Yamanobe Tomoyo
Central Laboratory Of Analytical Biochemistry School Of Medicine Teikyo University
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Nakamura Masahisa
Laboratory For Amphibian Biology Faculty Of Science Hiroshima University
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