Control of Growth and Differentiation of Chondrogenic Fibroblasts in Soft-Agar Culture : Role of Basic Fibroblast Growth Factor and Transforming Growth Factor-β
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概要
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The sclera of the chick embryo consists of a layer of cartilage cells (scleral chondrocytes) adjacent to a layer of perichondrium (chondrogenic fibroblasts), which can be separated to produce pure populations of each cell type. In soft-agar culture at low concentration of fetal bovine serum (FBS), basic fibroblast growth factor (bFGF) induced clonal growth of many undifferentiated fibroblast-type (F-type) colonies from chondrogenic fibroblasts. Under the same conditions, bFGF induced many differentiated cartilage-type (C-type) colonies from scleral chondrocytes. On the other hand, a high concentration (10%) of FBS induced many C-type colonies from the chondrogenic fibroblasts. These results indicate that bFGF induces the fibroblasts to proliferate without progression of differentiation, while FBS contains an activity which promotes cartilage differentiation of the fibroblasts. The proliferating fibroblasts retained their differentiative capacity for at least 20 days in culture. The bFGF-dependent proliferation of the chondrogenic fibroblasts was inhibited by low concentration of transforming growth factor-β (TGF-β). In contrast, with differentiated chondrocytes, TGF-β did not inhibit the bFGF-dependent proliferation, but promoted it synergistically. Conditioned medium harvested from protein-free monolayer cultures of chondrogenic fibroblasts contained a TGF-β-like molecule. These regulators may play roles in the growth and differentiation of chondrogenic cells in vivo.
- 社団法人日本動物学会の論文
- 1994-04-15
著者
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Ohya Yoshie
Cell And Developmental Biology Laboratory Faculty Of Integrated Arts And Sciences Hiroshima Universi
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Watanabe Kazuo
Cell And Developmental Biology Laboratory Faculty Of Integrated Arts And Sciences Hiroshima Universi