Amino Acid Flow through Aphid and Its Symbiont : Studies with ^<15>N-Labeled Glutamine

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Amino acid excretion of symbiotic and aposymbiotic pea aphids, Acyrthosiphon pisum was investigated using [ε-^<15>N]glutamine. Aphids were transferred to a synthetic diet containing [^<15>N]glutamine within 24hr after birth, and the honeydew was collected until they attained adulthood. While in the honeydew of symbiotic aphids the total content of ^<15>N remained less than 1.0 atom% excess, that in the honeydew of aposymbiotic ones gradually increased to about 6.0 atom% excess. The predominant amino acid in the former was arginine whose ^<15>N content was 0.60 atom% excess. Aposymbiotic aphids excreted great amounts of glutamine and asparagine, and the ^<15>N contents of these amino acids were 11.01 and 2.98 atom% excess, respectively. In addition to these amino acids, the honeydew of aposymbiotic aphids contained an unidentified, ninhydrin positive compound that was highly labeled with ^<15>N (6.4 atom% excess). This compound was identified as a mixture of γ-glutamylglutamine and γ-glutamylasparagine by analyses using NMR, MS and HPLC.
社団法人日本動物学会の論文
1993-10-15