Hemoglobin Transition from Larval to Adult Types in Normally Metamorphosing, Metamorphosed and Metamorphosis-Arrested Hynobius retardatus

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A transition of hemoglobin subunits during ontogeny of a hynobiid salamander, Hynobius retardatus, was studied using SDS-PAGE, AUT-PAGE and two-dimentaional electrophoresis in conbination of the two. Hemoglobins from normally metamorphosing and metamorphosed animals reared at different temperatures and from metamorphosis-arrested larvae, which were reared in aqueous solution of thiourea and sodium perchlorate, were analyzed . Larval type hemoglobin was composed of 4 electrophoretically distictive subunits, named HL1, HL2, HL3 and HL4. Similarly, adult hemoglobin was composed of 4 subunits, all of which were different from the larval subunits. Progress of the larval developmental stages basically depended on the temperature : the lower temperature caused retarded metamorphosis (10℃) or arrested metamorphosis (4℃). Transition of the hemoglobin in those animals also depended on the temperatures, but not on the ages or body sizes of them. Although no indication of metamorphosis was observed at external morphological level in the larvae reared at 4℃, adult type subunits of hemoglobin were detected in them. In metamorphosisarrested larvae reared at room temperature, the transition of hemoglobins occurred in the same time schedule as in the controls, suggesting that the transition was completely independent from the morphological metamorphosis.
社団法人日本動物学会の論文
1993-08-16