Toxicology of Maternally Ingested Carbon Tetrachloride (CCl_4) on Embryonal and Fetal Development and in vitro Fertilization in Mice

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Oral administration of carbon tetrachloride (CCl_4) for five consecutive days beginning on either day 1, 6 or 11 of pregnancy in mice had no effect on maternal body weight, liver and kidney weight or pregnancy. The various neonatal parameters e. g. pup weight and crown rump length were also not affected. No malformations were detected in any pup on day 1 post-partum. The development of pups (incisor eruption on day 11 and eye opening on day 14) was normal. CCl_4 at concentrations of 0.05 and 0.5 mM had no significant effect on in vitro fertilization while higher doses caused a significant decrease. A significant linear relationship between 1, 2, 5 and 10 mM CCl_4 and a decrease in the fertilization rate were found. Similarly all the doses of CCl_4 (1 to 10 mM) resulted in a significant increase in abnormal ovum forms. A significant linear relationship was found between the dose and percent of abnormal forms. Thus CCl_4 had no in vivo reproductive toxic effects following administration of 1 / 10 and 1/ 100 LD_<50> dose. However, addition of CCl_4 in culture medium in concentrations found in blood of lethally intoxicated rats, adversely affected the in vitro fertilization rate and caused an increased incidence in abnormal ova.
社団法人日本動物学会の論文
1993-02-15