Mouse Fibroblast Expressing Human Tyrosinase with DHICA-Oxidase Activity Produces Predominantly Pheomelanin Deposit in Lysosome

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概要

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• The melanogenic gene-transfected cell system serves as a useful tool for the study of the symphonic relation between melanin synthesis and intracellular organelles such as melanosomes in melanocytes. We constructed melanin-producing mouse fibroblasts by transfection of human tyrosinase cDNA to investigate the intracellular changes caused by tyrosinase expression. DHICA-oxidase (5,6-dihydroxyindole-2-carboxylic acid oxidase) activity without TRP-1 (Tyrosinase Related Protein-1) expression in the cells suggested that human tyrosinase also possesses a DHICA-oxidase activities different from mouse tyrosinase. Electron microscopic observation indicated that melanin-deposit organelles have some lysosomal features. These properties of melanin-deposit organelles in tyrosinase expressing fibroblasts provide one evidence for the hypothesis that melanosome is the specialized lysosome in melanocytes.

• 社団法人日本動物学会の論文
• 1996-12-15

著者

• MISHIMA Yutaka

  Mishima Institute for Dermatological Research

• Kondoh H

  Japan Sci. And Technol. Corp. (jst)

• Wilczek Adam

  Mishima Institute For Dermatological Research

• Kondoh Hirofumi

  Mishima Institute for Dermatological Research

• Narimizu Syuichi

  Sansyo Pharmaceutical Co. Ltd.

関連論文

• Biodistribution of Boron Concentration on Melanoma-bearing Hamsters after Administration of p-, m-, o-boronophenylalanine
• Huma Melanoma Treated by Boron Neutron Capture Therapy: Comparison of the Clinical Response with the Predicted Response
• Mouse Fibroblast Expressing Human Tyrosinase with DHICA-Oxidase Activity Produces Predominantly Pheomelanin Deposit in Lysosome

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