Changes in the Rigidity of the Hamster Egg during Meiotic Maturation and after Fertilization : Developmental Biology

概要

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When mature unfertilized hamster eggs were freed from their zonae pellucidae and centrifuged at 9,500×g, each egg elongated and then separated into light and heavy halves in about 10min. The rigidity of eggs at various stages of meiotic maturation and preimplantation development was examined by determining the minimum time needed for complete separation of all eggs in a group into halves or fragments . Eggs in the germinal vesicle stage were very rigid . They did not separate into halves even after 40min of centrifugation at 16,000×g. This rigidity appeared to he a property of the egg cortex rather than the endoplasm. The rigidity of the egg diminished sharply with the progression of meiotic maturation, reaching a minimum at metaphase of second meiosis, then increasing progressively after fertilization. Cytochalasin D reduced the rigidity of the egg regardless of the stage of meiotic maturation and development, indicating that the actin-based cytoskeleton in the cortical region is largely responsible for the rigidity of the egg.
社団法人日本動物学会の論文
1990-08-15