Embryo transfer and pregnancy rate in the golden hamster (Mesocricetus auratus) : Repreductive Biology

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TWO series of experiments were carried out to determine the reason for the low pregnancy rate after embryo transfer in the hamster. Two culture media, TC-199 and TALP plus 20% FCS, were tested for flushing and transfer of embryos. The 4- to 8-cell embryos were recovered from mated females (donors) and transferred to female recipients synchronized by the same hormonal regimen. The pregnancy rate after the use of TC-199 and TALP plus 20% FCS were similar, 36.4% and 39. 1% respectively, and compared with control females (80.0%). Only two of eight pregnant females in the TC-199 group and three of nine pregnant females in the TALP group delivered live young. The second series of experiments was carried out on three groups of females. Those in the first group were subjected to embryo transfer (ten, 4- to 8-cell embryos into right uterine horn). Those in the second groups were mated and received only medium in the right uterine horn. In the third group, also mated, sham injections were performed in the right uterine horn. All females were autopsied on Day 14 of gestation. The pregnancy rate in females of Groups I, II, and 111 were 50.0, 59.1, and 60.0% respectively, and the percent of pregnancies with at least one normal developed fetus from the right uterine horn of these three groups were 20.0, 46.2, and 58.3% respectively. In females of Groups 11 and III, the level of pregnancies in the right uterine horm were 30.8 and 17.7 percentage units less than in the left uterine horn. The number of normally developed fetuses in pregnant females of Group I was 24.4%, similar to that in the first experiment. The numbers of all recovered fetuses and of normally developed fetuses in the right uterine horn of females of Group 11 were significantly lower (25.8 and 19.5 respectively) than in the left uterine horn (74.2 and 56.6% respectively). A similar tendency was found in females of Group 111. Of 174 recovered fetuses from both uteri, 30.5% were from the right uterine Horn and 69.5% from the left uterine horn and the levels of normally developed fetuses were 14.9 and 55.2%, respectively. These results shown that the main reason for a decreased pregnancy rate after embryo transfer in the hamster is due to a trauma of the endometrium of the uterus and medium introduced into uteri, which may induce secretion of prostaglandins.
社団法人日本動物学会の論文
1990-02-15

Embryo transfer and pregnancy rate in the golden hamster (Mesocricetus auratus) : Repreductive Biology

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