Development of an In Vitro Spermiation System in the Frog, Rana nigromaculata : COMMUNICATION : Reproductive Biology

概要

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To clarify the mechanism of spermiation, particularly the release of spermatozoa from Sertoli cells, an in vitro spermiation system was developed. Testis pieces were incubated in various conditions, and then the degree of induction of release of spermatozoa from Sertoli cells was evaluated histologically. The induction of spermiation was seen in incubation for 1.5hr or longer at 18℃ following the addition of hCG (10 IU/ml) or bullfrog pituitary glycoprotein (GP: 5μg/ml). In this system, spermiation was not induced by the addition of insulin, arginine vasotosin (0.01-10μg/ml) or epinephrine (0.01-1μg/ml). These results suggest that the release of spermatozoa from Sertoli cells was induced specifically by the addition of gonadotropic substances in this system.
社団法人日本動物学会の論文
1989-10-15