Effect of ATPγS on Fertilization Envelope Elevation of Sand Dollar Eggs : COMMUNICATION : Developmental Biology
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概要
- 論文の詳細を見る
ATPγS (adenosine 5'-0-(3-thiotriphosph-ate)) inhibited cortical granule exocytosis of the sand dollar (Clypeaster japonicus) egg at insemination when injected before insemination at concentrations of 33-600μM in the cytoplasm . Accordingly the fertilization envelope did not elevate, whereas many sperm were incorporated into the egg and then sperm asters formed. After treatment with Ca ionophore A23187 the fertilization envelope did not elevate from eggs injected with ATPγS, although the threshold concentration of ATPγS to inhibit fertilization envelope elevation was higher than that at insemination. These results suggest that ATPγS would reduce Ca^<2+>-sensitivity of cortical granules by means of thiophosphorylation and, therefore, would prevent a transient increase in intracellular free Ca^<2+> concentration at insemination or parthenogenetic activation from inducing cortical granule exocytosis. Another analog, AMPPNP (5'-adenylylirnido diphosphate) (< 600μM in the egg cytoplasm) did not inhibit fertilization envelope elevation at insemination. However, ATPγS and AMPPNP at 900μM or more in the egg cytoplasm induced the envelope elevation by the injection. AMPPCP (5'-adenylylmethylene diphosphate) showed no effect.
- 社団法人日本動物学会の論文
- 1989-10-15
著者
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Hamaguchi Yukihisa
Biological Laboratory Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Hamaguchi Yukihisa
Biological Laboratory Tokyo Institute Of Technology
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Hamaguchi Yukihisa
Biological Laboratory Faclty Of Science Tokyo Institute Of Technology
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