Transient Increase in Mitochondrial Protein Synthesis of Starfish Embryo before Gastrulation : COMMUNICATION : Developmental Biology

概要

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A transient increase in protein synthesis in vitro was observed in mitochondrial fraction of starfish embryos at early gastrula stage. This stimulated activity was inhibited by chloramphenicol but not by cycloheximide. The purification of the protein synthesized in mitochondrial fraction was performed by column chromatography after labeling with [^3<H>]-amino acids and the peak fraction of radioactivity was analyzed by SDS-polyacrylamide gel electrophoresis . The result shows that there was only three species of newly synthesized proteins in mitochondrial fraction of starfish embryos. The molecular weights of these proteins were estimated about 62, 54 and 47 kD respectively.
1989-08-15